TY - JOUR AU - Gliniewicz, Karol AU - Wildung, Mark AU - Orfe, Lisa H. AU - Wiens, Gregory D. AU - Cain, Kenneth D. AU - Lahmers, Kevin K. AU - Snekvik, Kevin R. AU - Call, Douglas R. PY - 2015 DA - 2015/09/16 TI - Potential mechanisms of attenuation for rifampicin-passaged strains of Flavobacterium psychrophilum JO - BMC Microbiology SP - 179 VL - 15 IS - 1 AB - Flavobacterium psychrophilum is the etiologic agent of bacterial coldwater disease in salmonids. Earlier research showed that a rifampicin-passaged strain of F. psychrophilum (CSF 259-93B.17) caused no disease in rainbow trout (Oncorhynchus mykiss, Walbaum) while inducing a protective immune response against challenge with the virulent CSF 259–93 strain. We hypothesized that rifampicin passage leads to an accumulation of genomic mutations that, by chance, reduce virulence. To assess the pattern of phenotypic and genotypic changes associated with passage, we examined proteomic, LPS and single-nucleotide polymorphism (SNP) differences for two F. psychrophilum strains (CSF 259–93 and THC 02–90) that were passaged with and without rifampicin selection. SN - 1471-2180 UR - https://doi.org/10.1186/s12866-015-0518-1 DO - 10.1186/s12866-015-0518-1 ID - Gliniewicz2015 ER -