Table 4 Effect of different activators and inhibitors on the cholesterol oxidase activity of the enzyme produced by E. hirae
Enzyme | (U/ml) | |||
|---|---|---|---|---|
Activity | PPM | |||
Metal Ions | ||||
| Â | Control | 100 | 200 | 300 |
EDTA | 90 ± 0.01 | 82 + ±0.05 | 78 ± 0.04 | 70 ± 0.03 |
MgCl2 | 90 ± 0.03 | 91 ± 0.06 | 94 ± 0.03 | 84 ± 0.06 |
CoCl2 | 90 ± 0.02 | 78 ± 0.02 | 68 ± 0.05 | 61 ± 0.05 |
CdCl2 | 90 ± 0.01 | 81 ± 0.04 | 74 ± 0.03 | 65 ± 0.04 |
KCl | 90 ± 0.02 | 92 ± 0.03 | 83 ± 0.02 | 77 ± 0.03 |