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Table 2 Summary of purification steps

From: Purification and characterisation of the extracellular cholesterol oxidase enzyme from Enterococcus hirae

Purification steps

Enzyme activity (U/ml)

Protein content (mg/ml)

Specific enzyme activity (U/mg.protein)

Purification folds

Yield (%)

Cell free filtrate(crude)

129.60 ± 0.03

2.43 ± 0.06

53.30 ± 0.02

1.00

100

(NH4)2SO4 saturation level (80 %)

136.90 ± 0.05

1.45 ± 0.02

94.40 ± 0.04

1.80

105.70

Sephadex G-100

102.40 ± 0.02

0.82 ± 0.04

124.87 ± 0.02

2.30

79

  1. Difference between values is statistically significance level of 0.05