Fig. 6

SDS-PAGE analysis of the breakdown of whole human plasma by InpA. Plasma (1 μl, ~60μg protein), was incubated overnight at 37 °C in PBS, pH 7.4, containing 3 mM DTT in absence (lane 1) or presence (lane 2) of active InpA (100 μg ml⁻¹) in a final volume of 20 μl. ~ 50 μg total protein was loaded per lane. Arrow denotes albumin band. Samples were solubilised for 5 min at 100 °C in reducing application buffer, and gels were stained with CBB