Fig. 4From: Breakdown of albumin and haemalbumin by the cysteine protease interpain A, an albuminase of Prevotella intermedia Chemical reduction increases the susceptibility of albumin to InpA. Albumin (16 μM) was incubated with InpA (100 μg ml−1) at different pH at 37 °C under non-reducing (a panels) and reducing conditions (3 mM DTT) (b panels). Gel lanes had a nominal loading of 20 μg albumin, and are stained with CBB. Samples were prepared and gels run as described for Fig. 3 Back to article page