Skip to main content

Table 3 Oligonucleotide primers used for amplification in PCR

From: Spontaneous phenotypic suppression of GacA-defective Vibrio fischeri is achieved via mutation of csrA and ihfA

Primer

Sequence (5′ - 3′)

Annealing (°C)

Source

CsrA SOE A

CGCATTCAAGCGGAAACAGCAAAGAATGGCG

50

This Study

CsrA SOE B

CGCCATTCTTTGCTGTTTCCGCTTGAATGCG

50

This Study

CsrA SOE C

AGGTCCCTAGCGATAAGCCGGCTTCGCAAGGTA

50

This Study

CsrA SOE D

TACCTTGCGAAGCCGGCTTATCGCTAGGGACCT

50

This Study

RmsAF3

ATCACTAACCGCTGACCAAG

50

[17]

RmsAR4

AGCCAGGTACTCTATCCAGC

50

[17]

RmsAR7

TCACCGTCTTCAGGAGGC

50

[17]

Kan-2 FP-1

ACCTACAACAAAGCTCTCATCAACC

50

(Epicentre)

RmsAF8

TGTCGGTTATCGTAAATCAAGC

54

[17]

RmsAR9

AACCGTCTATGAAACGACCA

54

[17]

RsmB1F

AGTCAAAAGCGTAGT CTTATTGG

54

[17]

RsmB1R

TCACTGAGGAGAAATGTAACCG

54

[17]

RsmB2F

CTTACAAGCGAGTGAGATTTAGCG

56

[17]

RsmB2R

AGAGGAGGAACTGTATTGTGAGC

56

[17]

GacA4F

TAAATCGGAGTGTCAGTTGTG

51

[17]

GacA5R

AGGAAGGCACTACAGCGTC

51

[17]

QRpoBF1

CAAGAAGTAGATATTGCTGCTCTGT

57

This Study

QRpoBR2

AAGATTGGTGTAGCGATTGGTAA

57

This Study

QCsrAF1

CTGATGATTGGTGACGAAGGA

57

This Study

QCsrAR2

CGCATATAAATCTCTTCACGGTG

57

This Study