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Table 3 The oligonucleotides coding for wild type and truncated or mutant motifs

From: Identification of a novel linear epitope on the NS1 protein of avian influenza virus

Name The sequences of oligonucleotides Coding motifs
GST-wt-F 5'-GATCCTGGAATGATAACACAGTTCGAGTCTCTTAA C-3' WNDNTVRVS
GST-wt-R 5'-TCGAG TTAAGAGACTCGAACTGTGTTATCATTCCAG-3'  
GST-wtΔS-F 5'-GATCCTGGAATGATAACACAGTTCGAGTCTAA C-3' WNDNTVRV
GST-wtΔS-R 5'-TCGAG TTAGACTCGAACTGTGTTATCATTCCAG-3'  
GST-wtΔVS-F 5'-GATCCTGGAATGATAACACAGTTCGATAA C-3' WNDNTVR
GST-wtΔVS-R 5'-TCGAG TTATCGAACTGTGTTATCATTCCAG-3'  
GST-wtΔRVS-F 5'-GATCCTGGAATGATAACACAGTTTAA C-3' WNDNTV
GST-wtΔRVS-R 5'-TCGAG TTAAACTGTGTTATCATTCCAG-3'  
GST-wtΔVRVS-F 5'-GATCCTGGAATGATAACACATAA C-3' WNDNT
GST-wtΔVRVS-R 5'-TCGAG TTATGTGTTATCATTCCAG-3'  
GST-wtΔTVRVS-F 5'-GATCCTGGAATGATAACTAA C-3' WNDN
GST-wtΔTVRVS-R 5'-TCGAG TTAGTTATCATTCCAG-3'  
GST-wtΔW-VRVS-F 5'-GATCCAATGATAACACATAA C-3' NDNT
GST-wtΔW-VRVS-R 5'-TCGAG TTATGTGTTATCATTG-3'  
GST-N183S-F 5'-GATCCTGGTCTGATAACACATAA C-3' WSDNT
GST-N183S-R 5'-TCGAG TTATGTGTTATCAGACCAG-3'  
GST-D184G-F 5'-GATCCTGGAATGGAAACACATAA C-3' WDGNT
GST-D184G-R 5'-TCGAG TTATGTGTTTCCATTCCAG-3'  
GST-D184N-F 5'-GATCCTGGAATAATAACACATAA C-3' WNNNT
GST-D184N-R 5'-TCGAG TTATGTGTTATTATTCCAG-3'  
GST-T186S-F 5'-GATCCTGGAATGATAACTCATAA C-3' WNDNS
GST-T186S-R 5'-TCGAG TTATGAGTTATCATTCCAG-3'  
  1. Note: All primers expressing GST fusion proteins carried the BamHI restriction endonuclease site upstream and the XhoI site downstream, which are shown in italics. The stop codons are shown in bold