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Table 3 The oligonucleotides coding for wild type and truncated or mutant motifs

From: Identification of a novel linear epitope on the NS1 protein of avian influenza virus

Name

The sequences of oligonucleotides

Coding motifs

GST-wt-F

5'-GATCCTGGAATGATAACACAGTTCGAGTCTCTTAA C-3'

WNDNTVRVS

GST-wt-R

5'-TCGAG TTAAGAGACTCGAACTGTGTTATCATTCCAG-3'

 

GST-wtΔS-F

5'-GATCCTGGAATGATAACACAGTTCGAGTCTAA C-3'

WNDNTVRV

GST-wtΔS-R

5'-TCGAG TTAGACTCGAACTGTGTTATCATTCCAG-3'

 

GST-wtΔVS-F

5'-GATCCTGGAATGATAACACAGTTCGATAA C-3'

WNDNTVR

GST-wtΔVS-R

5'-TCGAG TTATCGAACTGTGTTATCATTCCAG-3'

 

GST-wtΔRVS-F

5'-GATCCTGGAATGATAACACAGTTTAA C-3'

WNDNTV

GST-wtΔRVS-R

5'-TCGAG TTAAACTGTGTTATCATTCCAG-3'

 

GST-wtΔVRVS-F

5'-GATCCTGGAATGATAACACATAA C-3'

WNDNT

GST-wtΔVRVS-R

5'-TCGAG TTATGTGTTATCATTCCAG-3'

 

GST-wtΔTVRVS-F

5'-GATCCTGGAATGATAACTAA C-3'

WNDN

GST-wtΔTVRVS-R

5'-TCGAG TTAGTTATCATTCCAG-3'

 

GST-wtΔW-VRVS-F

5'-GATCCAATGATAACACATAA C-3'

NDNT

GST-wtΔW-VRVS-R

5'-TCGAG TTATGTGTTATCATTG-3'

 

GST-N183S-F

5'-GATCCTGGTCTGATAACACATAA C-3'

WSDNT

GST-N183S-R

5'-TCGAG TTATGTGTTATCAGACCAG-3'

 

GST-D184G-F

5'-GATCCTGGAATGGAAACACATAA C-3'

WDGNT

GST-D184G-R

5'-TCGAG TTATGTGTTTCCATTCCAG-3'

 

GST-D184N-F

5'-GATCCTGGAATAATAACACATAA C-3'

WNNNT

GST-D184N-R

5'-TCGAG TTATGTGTTATTATTCCAG-3'

 

GST-T186S-F

5'-GATCCTGGAATGATAACTCATAA C-3'

WNDNS

GST-T186S-R

5'-TCGAG TTATGAGTTATCATTCCAG-3'

 
  1. Note: All primers expressing GST fusion proteins carried the BamHI restriction endonuclease site upstream and the XhoI site downstream, which are shown in italics. The stop codons are shown in bold