Fig. 7

cAMP-CRP specifically impacts gbpA expression in response to c-di-GMP. a Putative CRP-regulated genes were selected for analysis by qRT-PCR to assess transcript abundance in wild type (grey bars) and Δcrp (black bars) strains. The data were normalized relative to the wild-type, using rpoB and gyrA as the reference genes. b The transcript abundance for the genes analyzed in (a) was determined for V. cholerae with wild type (vector, grey bars) and reduced levels of c-di-GMP (pPDE, white bars). The data were normalized relative to the wild-type containing vector only, using rpoB and gyrA as the reference genes. a and (b) Shown are the means and standard deviations from at least three independent samples. For the indicated comparisons, ***P < 0.001 by unpaired t-test