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Table 5 Criteria for multivariate optimization of individual responses

From: Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents

Matrix

Response

Target

Lower limit

Upper limit

Weight

Importance

Manure

Δviable a

Target = 0

0.2

1.6

1

3

 

Δdead b

Target =4.1

2.4

4.1

1

3

Lagoon

Δviable

Target = 0

0.1

1.2

1

3

 

Δdead

Target =3.4

1.2

3.4

1

3

  1. adifference between the number of cultivable cells and the number of cells measured by qPCR after PMA treatment
  2. bdifference between the number of cultivable cells (before heat treatment) and the number of cells measured by qPCR after PMA treatment