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Table 4 Primers and probe used for L. monocytogenes quantification

From: Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents

Probe or primer Sequence (5′–3′) Denaturation temperature (°C)
Primers
 Forward TGC AAG TCC TAA GAC GCC A 60.3
 Reverse CAC TGC ATC TCC GTG GTA TAC TAA 60.3
Probe CGA TTT CAT CCG CGT GTT TCT TTT CG 70.2