Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents

Table 3 Physico-chemical parameters and Δ_viable and Δ_dead responses (expressed in log₁₀ cfu-eq) after PMA pretreatment of 5 manures and 4 lagoon effluents

Matrix	Sample	Δ_viable mean ± SD^b	Δ_dead ^a mean ± SD	pH	Turbidity (NTU)	VS (g.kg⁻¹)	TSS (g.kg⁻¹)
manure	M1	0.15 ± 0.2^C	2.97 ± 0.3^A	7.6	2612	6.3	3.8
	M2	0.83 ± 0.1^A	3.25 ± 0.0^A	8.2	2660	7.6	5.9
	M3	0.49 ± 0.2^ABC	2.51 ± 0.3^B	7.3	4624	22.8	14.8
	M4	0.29 ± 0.3^BC	2.40 ± 0.2^B	7.5	3323	19.8	12.9
	M5	0.21 ± 0.1^C	2.93 ± 0.2^A	7.6	3580	12.4	10.5
	Mean	0.39 ± 0.3	2.81 ± 0.4	7.7 ± 0.3	3360 ± 821	13.8 ± 7.3	9.6 ± 4.7
Lagoon	L6	0.12 ± 0.1^C	3.35 ± 0.0^A	8.5	102	1.6	0.9
	L7	0.36 ± 0.1^BC	3.35 ± 0.0^A	8.4	120	1.2	0.55
	L8	0.46 ± 0.1^BC	3.35 ± 0.0^A	8.4	461	1.8	1.1
	L9	0.69 ± 0.1^ABC	3.12 ± 0.3^A	8.4	772	1.3	1.0
	Mean	0.41 ± 0.2	3.29 ± 0.2	8.4 ± 0.0	364 ± 318	1.5 ± 0.3	0.9 ± 0.2

The values followed by different capital letters are statistically different according to a Student-Newman-Keuls test
^aThe concentrations of the inoculated cells were 7.4 log₁₀ cfu mL⁻¹ in the manure and 7.5 log₁₀ cfu mL⁻¹ in the lagoon, resulting in a maximum theoretical Δ_dead value of 3.2 and 3.5, respectively
^bmean of triplicates ± standard deviation

ISSN: 1471-2180