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Table 3 Physico-chemical parameters and Δviable and Δdead responses (expressed in log10 cfu-eq) after PMA pretreatment of 5 manures and 4 lagoon effluents

From: Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents

Matrix Sample Δviable mean ± SDb Δdead a mean ± SD pH Turbidity (NTU) VS (g.kg−1) TSS (g.kg−1)
manure M1 0.15 ± 0.2C 2.97 ± 0.3A 7.6 2612 6.3 3.8
  M2 0.83 ± 0.1A 3.25 ± 0.0A 8.2 2660 7.6 5.9
  M3 0.49 ± 0.2ABC 2.51 ± 0.3B 7.3 4624 22.8 14.8
  M4 0.29 ± 0.3BC 2.40 ± 0.2B 7.5 3323 19.8 12.9
  M5 0.21 ± 0.1C 2.93 ± 0.2A 7.6 3580 12.4 10.5
  Mean 0.39 ± 0.3 2.81 ± 0.4 7.7 ± 0.3 3360 ± 821 13.8 ± 7.3 9.6 ± 4.7
Lagoon L6 0.12 ± 0.1C 3.35 ± 0.0A 8.5 102 1.6 0.9
  L7 0.36 ± 0.1BC 3.35 ± 0.0A 8.4 120 1.2 0.55
  L8 0.46 ± 0.1BC 3.35 ± 0.0A 8.4 461 1.8 1.1
  L9 0.69 ± 0.1ABC 3.12 ± 0.3A 8.4 772 1.3 1.0
  Mean 0.41 ± 0.2 3.29 ± 0.2 8.4 ± 0.0 364 ± 318 1.5 ± 0.3 0.9 ± 0.2
  1. The values followed by different capital letters are statistically different according to a Student-Newman-Keuls test
  2. aThe concentrations of the inoculated cells were 7.4 log10 cfu mL−1 in the manure and 7.5 log10 cfu mL−1 in the lagoon, resulting in a maximum theoretical Δdead value of 3.2 and 3.5, respectively
  3. bmean of triplicates ± standard deviation