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Table 1 Design matrix of the Doehlert uniform shell design for 3 factors and corresponding responses

From: Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents

Run

Factors

Responses (log10 cfu-eq)

 

PMA concentration (μM)

Incubation time (min.)

Photoactivation time (min.)

Manure

Lagoon

    

Δviable

Δdead b

Δviable

Δdead

1a

160

17.5

29

1.2

3.4

1.0

3.4

2

160

17.5

29

0.9

3.4

1.0

3.4

3

160

17.5

29

1.1

3.4

1.0

3.4

4

160

17.5

29

1.1

3.4

1.0

3.4

5

300

17.5

29

1.6

4.1

1.2

3.4

6

20

17.5

29

0.2

2.7

0.1

3.4

7

230

17.5

56

1.3

4.1

1.2

3.4

8

90

17.5

2

0.6

2.4

0.4

2.5

9

230

17.5

2

1.2

2.4

0.3

2.5

10

90

17.5

56

0.6

3.1

1.0

3.4

11

230

30

38

1.0

4.1

0.6

3.4

12

90

5

20

0.6

2.9

0.3

1.2

13

230

5

20

1.2

3.3

0.7

3.4

14

160

5

47

0.8

4.1

0.8

3.4

15

90

30

20

0.5

3.2

0.7

3.4

16

160

30

11

1.0

4.1

0.6

3.4

  1. aThe central run (1 to 4) has been repeated 4 times in order to calculate residual variance
  2. bThe concentrations of the inoculated cells were 8.3 log10 cfu mL−1 in the manure and 7.3 log10 cfu mL−1 in the lagoon resulting in a maximum theoretical Δdead value of 4.1 and 3.4, respectively
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BMC Microbiology

ISSN: 1471-2180

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