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Table 1 Design matrix of the Doehlert uniform shell design for 3 factors and corresponding responses

From: Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents

Run Factors Responses (log10 cfu-eq)
  PMA concentration (μM) Incubation time (min.) Photoactivation time (min.) Manure Lagoon
     Δviable Δdead b Δviable Δdead
1a 160 17.5 29 1.2 3.4 1.0 3.4
2 160 17.5 29 0.9 3.4 1.0 3.4
3 160 17.5 29 1.1 3.4 1.0 3.4
4 160 17.5 29 1.1 3.4 1.0 3.4
5 300 17.5 29 1.6 4.1 1.2 3.4
6 20 17.5 29 0.2 2.7 0.1 3.4
7 230 17.5 56 1.3 4.1 1.2 3.4
8 90 17.5 2 0.6 2.4 0.4 2.5
9 230 17.5 2 1.2 2.4 0.3 2.5
10 90 17.5 56 0.6 3.1 1.0 3.4
11 230 30 38 1.0 4.1 0.6 3.4
12 90 5 20 0.6 2.9 0.3 1.2
13 230 5 20 1.2 3.3 0.7 3.4
14 160 5 47 0.8 4.1 0.8 3.4
15 90 30 20 0.5 3.2 0.7 3.4
16 160 30 11 1.0 4.1 0.6 3.4
  1. aThe central run (1 to 4) has been repeated 4 times in order to calculate residual variance
  2. bThe concentrations of the inoculated cells were 8.3 log10 cfu mL−1 in the manure and 7.3 log10 cfu mL−1 in the lagoon resulting in a maximum theoretical Δdead value of 4.1 and 3.4, respectively