Fig. 4

Hypovirulence of P. syringae pv. tomato DC3000 iaaL and matE mutants in tomato plants. a Competitive index (CI) values for mixed inoculations of P. syringae pv. tomato DC3000 and its derivate strains in tomato plants. Complementation was performed via ectopic expression of iaaL or matE cloned in the expression vector pJB3 (Table 1). Error bars indicate the standard error from the average of two different assays. Asterisks indicate CI values that were significantly different from one. Statistical analyses were performed using Student’s t-test and P = 0.005. b Representative images of symptoms induced by the indicated strains in var. Moneymaker tomato plants at 9 days post-inoculation (dpi). Mock, plants inoculated with a MgCl2 solution. c Quantification of necrotic areas in the leaflets shown in (b). The results are the means of nine different leaflets. Error bars represent the standard error