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Table 2 Strains, plasmids, and phages used in this study

From: The Escherichia coli NarL receiver domain regulates transcription through promoter specific functions

Strains

Parent

Genotype

Source

CJ236

 

F’ cat (pCJ105;M13SCmR)

[53]

  

dut ung thi relA spoT1 mcrA

 

JM109

 

endA1 recA1 gyrA96 hsdR17

[54]

  

(r k m k + ) relA1supE44 thi

 
  

[F traD36 proAB lacI q ZΔM15] Δ(lac-proAB)

 

MC4100

 

F Δ(argF-lac) U169 araD139

[44]

  

deoC1 relA1 flbB5301 rbsR rpsL50 ptsF25

 

MV1190

 

Δ(srl-recA)306:Tn10 (tetR)

BioRad

  

Δ(lac-proAB) thi supE

 
  

[F: traD36 proAB lacI qZΔM15]

 

RC11

MC4100

ΔnarXL ΔnarQ::kan recA56 srl::Tn10 tet

[46]

Phage

Parent

Genotype

Source

M13MK1

M13mp18

EcoRI-BamHl 'narX narL +

[46]

λLK1

λRS45

φ(frdA-lacZ) (hyb) lacY + lacA +

[55]

λPC51

λRS45

φ(narG-lacZ) (hyb) lacY + IacA +

[56]

Plasmid

Parent

Genotype

Source

pACYC184

 

CmR TcR

[47]

pLK63

pACYC184

narX + narL + Cm R

[45]

pMJ145

pLK63

narX + narL C (147–216)

This study

pMJ146

pLK63

narX + narL C (131–216)

This study

pMJ152

pLK63

narX + narL C (126–216)

This study

pMJ153

pLK63

narX + narL C (109–216)

This study

pMJ154

pLK63

narX + narL N (2–141)

This study

pQE9

 

ApR

Qiagen

pMJ05

pQE9

narL C (147–216)

This study

pMJ99

pQE9

narL N (2–141)

This study

pMJ104

pQE9

narL

This study