Table 2 Strains, plasmids, and phages used in this study

CJ236

F’ cat (pCJ105;M13^SCm^R)

[53]

dut ung thi relA spoT1 mcrA

JM109

endA1 recA1 gyrA96 hsdR17

[54]

(r _k ⁻ m _k ⁺ ) relA1supE44 thi

[F⁻ traD36 proAB lacI ^q ZΔM15] Δ(lac-proAB)

MC4100

F⁻ Δ(argF-lac) U169 araD139

[44]

deoC1 relA1 flbB5301 rbsR rpsL50 ptsF25

MV1190

Δ(srl-recA)306:Tn10 (tet^R)

BioRad

Δ(lac-proAB) thi supE

[F: traD36 proAB lacI ^qZΔM15]

RC11

MC4100

ΔnarXL ΔnarQ::kan recA56 srl::Tn10 tet

[46]

Phage

Parent

Genotype

Source

M13MK1

M13mp18

EcoRI-BamHl 'narX narL ⁺

[46]

λLK1

λRS45

φ(frdA-lacZ) (hyb) lacY ⁺ lacA ⁺

[55]

λPC51

λRS45

φ(narG-lacZ) (hyb) lacY ⁺ IacA ⁺

[56]

Plasmid

Parent

Genotype

Source

pACYC184

Cm^R Tc^R

[47]

pLK63

pACYC184

narX ⁺ narL ⁺ Cm ^R

[45]

pMJ145

pLK63

narX ⁺ narL ^{C (147–216)}

This study

pMJ146

pLK63

narX ⁺ narL ^{C (131–216)}

This study

pMJ152

pLK63

narX ⁺ narL ^{C (126–216)}

This study

pMJ153

pLK63

narX ⁺ narL ^{C (109–216)}

This study

pMJ154

pLK63

narX ⁺ narL ^{N (2–141)}

This study

pQE9

Ap^R

Qiagen

pMJ05

pQE9

narL ^{C (147–216)}

This study

pMJ99

pQE9

narL ^{N (2–141)}

This study

pMJ104

pQE9

narL

This study