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Table 2 Strains, plasmids, and phages used in this study

From: The Escherichia coli NarL receiver domain regulates transcription through promoter specific functions

Strains Parent Genotype Source
CJ236   F’ cat (pCJ105;M13SCmR) [53]
   dut ung thi relA spoT1 mcrA  
JM109   endA1 recA1 gyrA96 hsdR17 [54]
   (r k m k + ) relA1supE44 thi  
   [F traD36 proAB lacI q ZΔM15] Δ(lac-proAB)  
MC4100   F Δ(argF-lac) U169 araD139 [44]
   deoC1 relA1 flbB5301 rbsR rpsL50 ptsF25  
MV1190   Δ(srl-recA)306:Tn10 (tetR) BioRad
   Δ(lac-proAB) thi supE  
   [F: traD36 proAB lacI qZΔM15]  
RC11 MC4100 ΔnarXL ΔnarQ::kan recA56 srl::Tn10 tet [46]
Phage Parent Genotype Source
M13MK1 M13mp18 EcoRI-BamHl 'narX narL + [46]
λLK1 λRS45 φ(frdA-lacZ) (hyb) lacY + lacA + [55]
λPC51 λRS45 φ(narG-lacZ) (hyb) lacY + IacA + [56]
Plasmid Parent Genotype Source
pACYC184   CmR TcR [47]
pLK63 pACYC184 narX + narL + Cm R [45]
pMJ145 pLK63 narX + narL C (147–216) This study
pMJ146 pLK63 narX + narL C (131–216) This study
pMJ152 pLK63 narX + narL C (126–216) This study
pMJ153 pLK63 narX + narL C (109–216) This study
pMJ154 pLK63 narX + narL N (2–141) This study
pQE9   ApR Qiagen
pMJ05 pQE9 narL C (147–216) This study
pMJ99 pQE9 narL N (2–141) This study
pMJ104 pQE9 narL This study