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Table 1 Summary of sedimentation equilibrium resultsa,b

From: The Escherichia coli NarL receiver domain regulates transcription through promoter specific functions

Construct Sequence MMc (kDa)c Speed (krpm)c Measured MM at 60 μM (kDa) Measured MM at 160 μM (kDa) Predominant Oligomeric State
NarLC (147–216) 9.6 15 9.7 12.3 Monomer
   22 9.6 11.4  
NarLC (126–216) 11.8 15 14.1 13.6 Monomer
   22 12.7 12.4  
NarL 23.9 11 24.0 23.9 Monomer
   15 22.6 23.0  
NarLN 16.5 15 26.1 25.9 Monomer/Dimer
   22 23.4 23.0  
NarLN-P 16.6 15 30.8 30.4 Dimer
   22 29.0 27.4  
  1. aCertain constructs had additional data collected at other speeds that are not shown in the table but were included in determining the best oligomeric fit (discussed in text)
  2. bAll runs shown were carried out in a buffer solution containing 25 mM Tris, pH 7.5-8.5 and 500 mM NaCl, except for two cases: NarLC (147–214) at 60 μM contained 500 mM (NH4)2SO4, and NarL at 60 μM contained 100 mM NaCl. These changes to salt did not significantly alter the measured molecular masses (see Materials and Methods)
  3. cMM: molecular mass; kDa: kiloDalton; krpm: kilorevolutions per minute