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Fig. 5 | BMC Microbiology

Fig. 5

From: The Escherichia coli NarL receiver domain regulates transcription through promoter specific functions

Fig. 5

Representative sedimentation equilibrium plots. Plots are single exponential fits with the residuals shown in the upper plot of each panel. Triangles represent unphosphorylated proteins, circles represent phosphorylated proteins. Samples shown were run at a concentration of 60 μM and the measured molecular-masses in kiloDalton (kDa) are indicated. (a) NarLN and NarLN-P after reaching equilibrium at 15,000 rpm and 240 nm. The sequence determined molecular-mass of NarLN is 16.5 kDa, therefore NarLN exists as a monomer and dimer while NarLN-P is mostly a dimer. (b) NarLC (126–216), with a sequence-determined molecular mass of 11.8 kDa, after reaching equilibrium at 22,000 rpm and 280 nm. This construct, as with the shorter NarLC (147–216) construct, is predominantly a monomer

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