Fig. 3
DNase I protection patterns for NarLC (147–216), NarL, and NarL-P. Binding to a 391 base pair fragment containing the narG promoter region is shown. The vertical bars indicate the two major regions of protection seen for NarL-P, which are not as strong or as extensive in NarLC (147–216). Dotted lines highlight areas of protection. Asterisks indicate altered banding patterns or hypersensitive bands for NarL-P. Arrows indicate altered banding patterns for NarLC (147–216). Maxam-Gilbert G-reactions performed on the same DNA fragment were used as size markers. Coordinates relative to the transcription start site are given in base-pairs, and protein concentrations are indicated