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Fig. 7 | BMC Microbiology

Fig. 7

From: Evidence for the involvement of cofilin in Aspergillus fumigatus internalization into type II alveolar epithelial cells

Fig. 7

β-1,3-glucan signalling was not associated with the cofilin phosphorylation induced by A. fumigatus. A549 cells were stimulated with the indicated concentrations of β-1,3-glucan (100 ug/ml) for the indicated times. Thereafter, the level of cofilin and p-cofilin were detected by western blotting (a). Other A549 cells were first incubated with complete DMEM media, complete DMEM supplemented with 5 μg/ml of isotype control antibody (IgG1) or complete DMEM supplemented with 5 μg/ml of anti-dectin-1 mAb (ab82888) for 30 min. Then, they were infected with the swollen conidia of A. fumigatus 13073 at an MOI of 10 for 30 min. Finally expression of cofilin/p-cofilin was analysed by western blotting (c). The densitometric analyses of the western blots for three independent experiments are shown (b, d). * P < 0.05, and n.s. represent no difference between the columns. Blots are characteristic of three independent experiments

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