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Fig. 6 | BMC Microbiology

Fig. 6

From: Evidence for the involvement of cofilin in Aspergillus fumigatus internalization into type II alveolar epithelial cells

Fig. 6

ROCK and RhoA were involved in the regulation of A. fumigatus invasion and cofilin phosphorylation. A549 cells were treated with Y27632 (a specific inhibitor of ROCK) at the indicated concentration for 1 h (a, b) or transfected with pEF-C3T or the empty vector (control) for 36 h (c, d) prior to inoculation with A. fumigatus. The cells were inoculated with swollen conidia for 1 h at an MOI of 10. Then, A. fumigatus internalization was analysed by the nystatin protection assay (a, c). The expression of cofilin and p-cofilin as measured by western blotting with the indicated antibodies. The densitometric analyses of the western blots are shown (B, D). The means ± SE of three independent experiments are shown. *P < 0.05 compared with 0 or control. Blots are characteristic of three independent experiments

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