Fig. 5

LIMK and SSH modulated the phosphorylation cycle of cofilin to affect the internalization of A. fumigatus swollen conidia. A549 cells were either treated with BMS-5 (the pharmacological inhibitor of LIMK) at the indicated doses for 1 h or transfected with pCDNA3.1/myc-tagged-SSH1L or the empty vector (control) for 36 h prior to inoculation with A. fumigatus. The cells were inoculated with swollen conidia for 1 h at an MOI of 10; then, the internalization and protein levels were analysed. A. fumigatus internalization was analysed by the nystatin protection assay (a, c). The expression of cofilin and p-cofilin were measured by western blotting with the indicated antibodies. The densitometric analyses of the western blots are shown (b, d). The means ± SE of three independent experiments are shown. * P < 0.05 compared with 0 or the control. Blots are characteristic of three independent experiments