Effect of the synthetic cannabinoid HU-210 on quorum sensing and on the production of quorum sensing-mediated virulence factors by Vibrio harveyi

Table 3 Relative mRNA expression of different genes in V. harveyi

Relative mRNA expression	BB120	MM30	BB152
with HU-210 (2 μg/ml)	RQ value
luxS	0.81 ± 0.03	-	0.42 ± 0.05*
luxP	0.98 ± 0.45	-	0.37 ± 0.25
luxQ	1.1 ± 0.65	-	0.36 ± 0.11
luxM	3.05 ± 0.46	4.04 ± 1.42	-
luxN	1.31 ± 0.31	2.34 ± 0.87	-
luxR	0.45 ± 0.12	1.34 ± 0.19	0.43 ± 0.11*

Relative expression of genes was measured using real-time RT-PCR as described in Materials and Methods. Fold change of the transcriptional level was calculated using ABI-Prism 7300 software v1.1 with RQ study 1.0, Applied Biosystems. The expression results represent mean ± SD of two independent experiments. *P < 0.05

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