|
Relative mRNA expression
|
BB120
|
MM30
|
BB152
|
|---|
|
with HU-210 (2 μg/ml)
|
RQ value
|
|
luxS
|
0.81 ± 0.03
|
-
|
0.42 ± 0.05*
|
|
luxP
|
0.98 ± 0.45
|
-
|
0.37 ± 0.25
|
|
luxQ
|
1.1 ± 0.65
|
-
|
0.36 ± 0.11
|
|
luxM
|
3.05 ± 0.46
|
4.04 ± 1.42
|
-
|
|
luxN
|
1.31 ± 0.31
|
2.34 ± 0.87
|
-
|
|
luxR
|
0.45 ± 0.12
|
1.34 ± 0.19
|
0.43 ± 0.11*
|
- Relative expression of genes was measured using real-time RT-PCR as described in Materials and Methods. Fold change of the transcriptional level was calculated using ABI-Prism 7300 software v1.1 with RQ study 1.0, Applied Biosystems. The expression results represent mean ± SD of two independent experiments. *P < 0.05
