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Fig. 4 | BMC Microbiology

Fig. 4

From: Effect of the synthetic cannabinoid HU-210 on quorum sensing and on the production of quorum sensing-mediated virulence factors by Vibrio harveyi

Fig. 4

Analysis of V. harveyi biofilms by CLSM. (a) Comparison of biofilm depth of V. harveyi BB120 (wild type), MM30 (AI-1+, AI-2) and BB152 (AI-1, AI-2+) formed with or without different concentrations of HU-210. Presented data are means and SD of three independent experiments.*P < 0.05 compared with control. (b) V. harveyi BB120 (wild type), MM30 (AI-1+, AI-2) and BB152 (AI-1, AI-2+) biofilms grown with or without HU-210 (200 μg/ml) and stained with SYTO 9 dye (green), which stains live bacteria, and with concanavalin A–Alexa Fluor 647 conjugates (blue), which stain extracellular polysaccharides. Biofilm depth of V. harveyi BB120 (wild type), MM30 (AI-1+, AI-2) and BB152 (AI-1, AI-2+) biofilms in control (without HU-210) is 85 μm, 80 μm ,95 μm and in samples with HU-210 (200 μg/ml) is 80 μm, 85 μm, 55 μm respectively

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