Fig. 4

Peptide inhibition of the translocator:chaperone interaction. (a) Recombinant GST-CopD_1–157 or GST-CopB_1–200 was pre-incubated with 500 μM of the chaperone binding domain peptide mimetic (+) or vehicle alone (−). CopD_1–157 and GST-CopB_1–200 did not interact with its putative chaperone in the presence of the CBD peptide, but did so in the presence of a control peptide or vehicle alone. (b) Left image is C. pneumoniae incubated with vehicle alone (PBS), right image is C. pneumoniae incubated with 500 μM CBD Peptide, bottom image is C. pneumonia incubated with 50 uM anti-RSV peptide. Chlamydial inclusions are stained green, while HeLa cells are stained red by Evan’s blue counterstain