Fig. 3

LcrH_1 (Cpn0811) interacts with CopB. (a) Recombinant LcrH_1 interacted with amino acids 1–200 of CopB. CopB mutants were created using Gblock synthesis: ^P166ACopB_1–200, ^L168ACopB_1–200, and ^P171ACopD_1–200. (b & c) Mutations at the conserved amino acids within the predicted chaperone binding domain disrupted the interaction between CopB_1–200 and the chaperone LcrH_1, but not other identified interactions