Fig. 5
From: Portability of the thiolation domain in recombinant pyoverdine non-ribosomal peptide synthetases
Location of T domains within Pseudomonas pyoverdine NRPS gene clusters that were used to substitute the T1 domain of pvdD. a Domain architecture of the four NRPS genes involved in P. aeruginosa PAO1 pyoverdine synthesis. (b) Domain architecture of genes involved in the synthesis of pyoverdine variable peptide chains from three other Pseudomonas strains. Location and numbering of the six TC (blue), four TCt (red), four TE (green), and four TTE (purple) domains used for domain substitution are indicated by the colour-coded shaded boxes. Standard abbreviations are used to identify the amino acid substrate incorporated by each module, with the following additions: Dab, 2,4-diaminobutyric acid; fhOrn, L-N5-formyl-N5-hydroxyornithine; hOrn, N5-hydroxyornithine. Within this figure the three modules from which domains were sourced for C-A and T-C-A domain substitution are identified by rectangles around the name of the substrate activated by that module as well as addition of numbers after that substrate name, to correspond with the nomenclature used in the C-A and T-C-A domain substitutions