Antimicrobial activities of actinomycetes isolated from unexplored regions of Sundarbans mangrove ecosystem

BMC Microbiology

Table 2 Antibacterial and Antifungal activity of crude ethyl acetate extracts of SMS_7, SMS_SU13 and SMS_SU21

Test organism	SMS_7		SMS_SU13		SMS_SU21
	Zone of inhibition (mm)	MIC (mg ml⁻¹)	Zone of inhibition (mm)	MIC (mg ml⁻¹)	Zone of inhibition (mm)	MIC (mg ml⁻¹)
Escherichia coli (ATCC 25922)	++	5	+++	5	+++	0.5
Escherichia coli (ATCC 8739)	++	5	++	0.5	++	0.05
Staphylococcus aureus (ATCC 25923)	++	0.5	+	5	++++	0.05
Bacillus subtilis (ATCC 6633)	++	0.5	+	5	+++	0.5
Pseudomonas aeruginosa (ATCC 27853)	+	0.5	++++	0.05	+++	0.05
Enterobacter aerogenes (ATCC 13048)	++	5	++	5	+++	0.5
Salmonella typhi (ATCC 6539)	+	5	+++	0.5	+++	0.5
Salmonella Typhimurium (ATCC 14028)	+	5	+++	0.5	++	0.5
Vibrio cholerae (MTCC 3906)	+++	0.5	+++	0.5	++++	0.05
Saccharomyces cerevisiae (ATCC 9763)	+++	0.05	-	-	+	5
Candida albicans (ATCC 10231)	++	0.5	+	5	++	0.5
Aspergillus niger (ATCC 16404)	+++	0.05	++	5	+++	0.5
Ustilago maydis SG200	+++	0.05	++	5	++	0.5
Macrophomina phaseolina R9	+++	0.5	+	5	++	5
Rhizoctonia soloni AG1-1A	++	0.5	+	5	++	0.5

The level of growth inhibition was assessed by the average zone of inhibition diameter recorded (4 replicates). ‘++++’ = zone of inhibition ≥ 25 mm; ‘+++’ = zone of inhibition ≥ 20–24 mm; ‘++’ = zone of inhibition ≥ 10–19 mm; ‘+’ = zone of inhibition ≥ 5–9 mm; and ‘-’ = no zone of inhibition. [Well diameter (5 mm) was included]. MIC was defined as the lowest concentration that produced zone of inhibition ≥5 mm in diameter against test organism

ISSN: 1471-2180