From: Comparative analysis of multiple inducible phages from Mannheimia haemolytica
No. (Gene name) | Size (aa)/MW (kDa)/pI | Function | Motifsa | Best homologs | % identity (aa) to Aaphi23b | Absent in phages |
---|---|---|---|---|---|---|
1 terS | 174/19.1/5.4−5.6 | Terminase, small subunit | Terminase_2 (pfam03592) | Bacteriophage terminase small subunit (M. haemolytica) | 52−54 |  |
2 terL | 406−410/46.3−47.3/5.9−6.1 | Terminase, large subunit | Terminase_3 superfamily (cl12054) | Phage terminase, large subunit, PBSX (M. haemolytica) | 19−21 |  |
3 porT | 458−467/50.6−52.2/4.9−5.1 | Portal protein | phage_prot_Gp6 (pfam05133) | Hypothetical protein (M. haemolytica) | 20−25 |  |
4 MHP | 312−553/35.4−63.3/9.3−9.5 | Head morphogenesis protein | Phage_Mu_F superfamily (cl10072) | Hypothetical protein (M. haemolytica) | 12−23 |  |
5 MTP | 160/17.3/5.1 | Major tail protein | Phage_tail_2 (pfam06199) | Phage major tail protein (M. haemolytica) | Â | 1152AP2 |
6 | 137/15.1/10.2 | Hypothetical protein | DUF4128 (pfam13554) | Hypothetical protein (M. haemolytica) | Â | 535AP2, 3927AP1, 587AP2 |
7 hicB | 138/15.5/4.6 | HicB | UPF0150 (pfam03681) | Toxin-antitoxin , antitoxin component, HicB (M. haemolytica) | Â | 1152AP2 |
8 hicA | 58/6.6/10.1 | HicA | YcfA (pfam07927) | Toxin-antitoxin, toxin component, HicA (M. haemolytica) | Â | 1152AP2 |
9 TMP | 816−1008/88.1−108.8/5.2−6.2 | Tail length tape measure protein | Tape_meas_nterm superfamily (cl15680); TMD(1) | Tail length tape measure-related protein (M. haemolytica) | 17−19 |  |
10 M | 107−109/12.3−12.5/8.8−9 | Minor tail protein M | Phage_min_tail (pfam05939) | Gifsy-1 prophage VmtM (M. haemolytica) |  |  |
11 L | 156/17.6/5 | Minor tail protein L | Phage_tail_L superfamily (cl01908) | Phage-related minor tail protein L (M. haemolytica) | Â | 1152AP2 |
12 L | 238/26.5/6−6.3 | Minor tail protein L | Phage_tail_L superfamily (cl01908) | Phage minor tail protein L (M. haemolytica) |  |  |
13 K | 243/28.5/5.3 | Minor tail protein K | MPN_NLPC_P60 (cd08073) | Bacteriophage tail protein (M. haemolytica) | Â | Â |
14 I | 196−209/20.9−22.1/9.6−9.9 | Tail assembly protein I | Lambda_tail_I superfamily (cl01945); TM(2) | Bacteriophage tail protein and tail assembly protein I (M. haemolytica) | 15−19 |  |
15 J | 1954−2352/213.3−255/6.3−8 | Host specificity protein J | Phage-tail_3 (pfam13550); TM(2) | Host specificity protein J (M. haemolytica) |  |  |
16 int | 329−351/37.9−40.8/9.7−9.9 | Integrase | Phage_integrase (pfam00589); Phage_integ_N superfamily (cl07565); DNA_BRE_C superfamily (cl00213) | Integrase/recombinase (M. haemolytica) | 17−24 |  |
17 MTase | 163/19.2/8.3 | Methyltransferease | Methyltransf_25 (pfam13649) | Putative bacteriophage methyltransferase (M. haemolytica) | Â | 3927AP1, 1152AP2, 587AP2 |
18 | 127/14.6/5.6 | Hypothetical protein | flap endonuclease-1-like (cl14815) | Hypothetical protein (M. haemolytica) | Â | 535AP2, 587AP2 |
19 ant | 218−233/26−26.9/7.1−7.7 | Antirepressor protein Ant | P22_AR_N (pfam10547); P22_AR_C superfamily (cl11179); AntA superfamily (cl01430) | Antirepressor protein Ant (M. haemolytica, Avibacterium paragallinarum for 587AP2) | 19−21 | 535AP2 |
20 | 71/8/9.9 | Hypothetical protein | PRK11675 superfamily (cl08198) | Hypothetical protein (M. haemolytica) | Â | 535AP2, 587AP2 |
21 | 76/8.8/4.5 | Hypothetical protein | KilA-N (pfam04383) | KilA-N domain-containing protein (M. haemolytica) | Â | 535AP2, 587AP2 |
22 HNH | 168/19.4/9.6 | HNH homing endonuclease | HNH_3 (pfam13392) | Putative HNH endonuclease (Lactococcus) | Â | 535AP2, 3927AP1, 1152AP2 |
23 MTase | 198/21.3/8.3 | Cytosine-specific DNA methyltransferease | Cyt_C5_DNA_methylase superfamily (cl18939) | Cytosine-specific methyltransferase (Haemophilus parasuis) | Â | 535AP2, 3927AP1, 1152AP2 |
24 PK | 150/17.1−17.3/4.8−5 | Pyruvate kinase |  | Hypothetical protein and pyruvate kinase (M. haemolytica) |  |  |
25 exo | 211−225/23.5−25.9/ 4.8−5.9 | Exonuclease | YqaJ (pfam09588) | Bacteriophage exonuclease (M. haemolytica) |  |  |
26 bet | 264−307/29.2−35.3/5.2−5.3 | Recombinase | RecT (pfam03837); bet_lambda (TIGR01913) | Bet protein (M. haemolytica) |  |  |
27 | 154/17.9/6.5 | Hypothetical protein | NTP-PPase_u3 (cl16941) | Hypothetical protein (M. haemolytica) | Â | Â |
28 higB | 91/10.8/6.9 | HigB | HigB (COG3549) | Plasmid maintenance system killer (M. haemolytica) | Â | 587AP2 |
29 higA | 101/11.4/8.1 | HigA | Antidote_HigA (TIGR02607) | Plasmid maintenance system antidote protein (M. haemolytica) | Â | 587AP2 |
30 TRase | 346/39.6/8.8 | Transposase | HTH_28 (pfam13518) | Transposase (M. haemolytica) | Â | 587AP2 |
31 xhlA | 161/18.5/5.5−5.8 | Hypothetical protein | XhlA (pfam10779); TM (1) | Hypothetical protein (M. haemolytica) |  |  |
32 cI | 219−228/24.8−26.3/4.9−5.5 | CI repressor | S24_LexA-like (cd06529) | LexA family repressor/S24 family protease (M. haemolytica); bacteriophage transcriptional regulator (Haemophilus parasuis, for 587AP2) | 20−24 |  |
33 cro | 68−90/7.5−10.1/6.1−9.1 | Cro repressor | HTH_XRE (cd00093) | XRE family transcriptional regulator (M. haemolytica) | 14−28 |  |
34 cII | 86/9.8/8 | CII protein | Â | Bacteriophage CII protein (M. haemolytica) | 23 | 587AP2 |
35 | 80/9/9.6 | Hypothetical protein | HTH_39 (pfam14090) | Hypothetical protein (M. haemolytica) | Â | 535AP2, 3927AP1, 1152AP2 |
36 O | 276−289/31.8−33.3/8.9−9.1 | Replication protein O | Phage_rep_O (pfam04492) | Bacteriophage replication protein (M. haemolytica) | 18−21 | 587AP2 |
37 P | 215/24.9/9.2 | Replication protein P | Phage_lambda_P superfamily (cl06169) | Putative bacteriophage replication protein (M. haemolytica) | 13 | 535AP2, 3927AP1, 1152AP2 |
38 hel | 453/50.9/5.7 | Helicase | DnaB (TIGR00665) | Replicative DNA helicase (M. haemolytica) | Â | 587AP2 |
39 MTase | 178/20.7/8.8 | Methyltransferase | MT-A70 (pfam05063) | Hypothetical protein and modification methylase MunIM (M. haemolytica) | Â | 587AP2 |
40 MTase | 190/21.6/5 | DNA N-6-adenine-methyltransferas | Dam superfamily (cl05442) | DNA N-6-adenine methyltransferase (M. haemolytica) | Â | 535AP2, 3927AP1, 1152AP2 |
41 | 150−168/17.7−19.9/6.1−8.6 | Hypothetical protein | DUF1367 superfamily (cl06231) | Hypothetical protein (M. haemolytica) |  |  |
42 ninG | 189/22.3/9.7 | NinG protein | NinG (pfam05766) | Protein NinG (M. haemolytica) | 43 | 587AP2 |
43 | 94/10.6/9 | Hypothetical protein | DUF1364 (pfam07102) | Hypothetical protein (M. haemolytica) | Â | 535AP2, 3927AP1, 1152AP2 |
44 RusA | 122/13.9/9.4 | Endodeoxyribonuclease RusA | RusA (pfam05866) | Endodeoxyribonuclease RusA (Haemophilus influenzae) | Â | 535AP2, 3927AP1, 1152AP2 |
45 Q | 121−157/14.3−18.6/9.2−9.5 | Antitermination protein Q | Phage_antitermQ (pfam06530) | Phage anti termination protein Q (M. haemolytica) | 18−22 |  |
46 S | 81−117/9.5−12.8/9−9.3 | Holin | Phage_holin_3 superfamily;TM (1–3) | Hemophilus-specific protein (M. haemolytica) | 11−17 |  |
47 R | 189−197/21.2−22.3/9.1−9.5 | Endolysin | endolysin_autolysin (cd00737); lysozyme_like superfamily (cl00222); TM (1) | Lysozyme (M. haemolytica) | 21−55 |  |
48 Rz | 116/13.2/7.8−8.6 | Lytic protein Rz | DUF2570 (pfam10828); TM (1) | Hypothetical protein (M. haemolytica) | 34−36 |  |
49 Rz1 | 57−74/6.6−8.4/5−8 | Lytic protein Rz1 |  | Hypothetical protein (M. haemolytica) | 30−44 |  |