Development of a heptaplex PCR assay for identification of Staphylococcus aureus and CoNS with simultaneous detection of virulence and antibiotic resistance genes

Table 1 Oligonucleotide primers used in the heptaplex PCR assay

Target DNA	Amplicon size (bp)	Primer Identity	Primer sequence 5’ → 3’	Reference
cns	204	cns-1	TATCCACGAAACTTCTAAAACAACTGTTACT	[27]
cns	204	cns-2	TCTTTAGATAATACGTATACTTCAGCTTTGAATTT	[27]
16S rRNA	174	16S-1	CTAGTAATCGCGGATCAGCAT	[27]
16S rRNA	174	16S-2	GATACGGCTACCTTGTTACGACTT	[27]
mecA	155	mecA-1	TGGTATGTGGAAGTTAGATTGGGAT	[26]
mecA	155	mecA-2	CTAATCTCATATGTGTTCCTGTATTGGC	[26]
tuf	143	tuf-1	TACCAGCATTAGTAGTATTCTTAAACAAAGTTG	This study
tuf	143	tuf-2	TGCTGAACCAGCGATTACAG	This study
pvl	118	pvl-1	TTACACAGTTAAATATGAAGTGAACTGGA	[26]
pvl	118	pvl-2	AGCAAAAGCAATGCAATTGATG	[26]
vanA	111	vanA-1	GCTGTGAGGTCGGTTGTG	This study
vanA	111	vanA-2	GCTCGACTTCCTGATGAATACG	This study
spa	101	spa-1	CAGCAAACCATGCAGATGCTA	[26]
spa	101	spa-2	CGCTAATGATAATCCACCAAATACA	[26]

ISSN: 1471-2180