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Fig. 2 | BMC Microbiology

Fig. 2

From: Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA

Fig. 2

The PCR target region. Multiple sequence alignment of variants of the PCR target sequence. The positions of the primers and probe targets are highlighted in green and yellow respectively. Dots indicate identity to the prototype sequence. Variant nucleotides are shown as highlighted letters. Blue highlighting indicates variations that make non-destabilizing G:T base pairing with the primer/probe. Red highlighting indicates destabilizing mismatches. Variant nucleotides outside the primer/probe targets are highlighted in grey. *: isolates of exclusively far-eastern origin

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