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Fig. 4 | BMC Microbiology

Fig. 4

From: Leukocyte presence does not increase microbicidal activity of Platelet-rich Plasma in vitro

Fig. 4

Klebsiella pneumoniae growth inhibition. Each bacterial dilution to be tested (104, 105, 106 CFU/ml, as reported above each panel) was incubated with L-PRP, L-PRP cryo and P-PRP at 37 °C for 1, 2, 4 h. Then each bacterial suspension was spread on Blood Horse Agar plates and incubated at 37 °C for 18–24 h. A semi quantitative estimation of bacterial growth (CFU/ml) was performed by counting the colonies in each plate quadrant (for details see Methods). The antimicrobial activity was calculated as difference between experimental conditions and control culture conditions and expressed as growth inhibition: 1log, 2log, 3log and 4log growth inhibition indicate a decrease of 10, 100, 1000 and 10000 CFU/ml respectively compared to CFU/ml indicated above each panel. Data are reported as percentage of subjects (Frequency %) displaying a certain inhibition. Growth inhibition differences among incubation times, within equal PRP preparations and bacterial concentrations. Friedman-Anova test: a-h) not significant; i) p < 0.02. Wilcoxon-matched pair test: not significant. Growth inhibition differences among bacterial concentrations, within equal incubation times and PRP preparations. Friedman-Anova test: 1 h a) vs b) vs c) and g) vs h) vs i) p < 0.05; d) vs e) vs f) not significant; 2 h a) vs b) vs c) and g) vs h) vs i) p < 0.01; d) vs e) vs f) p < 0.05; 4 h a) vs b) vs c) p < 0.01; d) vs e) vs f) p < 0.05; g) vs h) vs i) not significant. Wilcoxon-matched pair test: 2 h g) vs i) p < 0.01. Growth inhibition differences among PRP preparations within equal bacterial concentrations and incubation times. Friedman-Anova test: a) vs d) vs g) 1 h p < 0.02; 2 h p < 0.05; b) vs e) vs h) 1 h p < 0.05; c) vs f) vs i) 1 h p < 0.05. Wilcoxon-matched pair test: not significant

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