Fig. 5

Kinetics of viral load levels in various tissues of EV-A71CC063-infected (10^6.5 CCID₅₀/ml) one-day-old ICR mice. The viral RNA copies were detected by qRT-PCR at days 2, 4, and 6 post-infection, respectively. The results represent the mean virus loads [(log₁₀copies)/mg of tissue or/ml of blood] ± SD (3 mice per group, repeated 3 times). RNA samples from the mock-infected mice and positive virus culture medium were run simultaneously in each qRT-PCR, and known copies of the DNA fragments were used as standards to calculate the copy numbers of the viral RNA in the infected tissues