Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Helicobacter pylori HP0377, a member of the Dsb family, is an untypical multifunctional CcmG that cooperates with dimeric thioldisulfide oxidase HP0231

Name

Genotype or relevant characteristics

Origin

Helicobacter pylori strains

26696

H. pylori wild-type

ATCC

N6

H. pylori wild-type

[24]

PR305

N6 dsbI::aph

[31]

PR336

dsbI + in trans complementant of dsbI::aph

[31]

PR378

N6 hp0231::cat

[31]

PR397

hp0231 + in trans complementant of hp0231::cat

[31]

MG100

N6 hp0265::aph

This study

Escherichia coli strains

BL21 (DE3)

F- ompT hsdSB(rB-mB-) gal dcm lon

Novagen

Rosetta (DE3) LacIq

F- ompT hsdSB (rB- mB-) gal dcm pRARE (Cmr)

Novagen

TG1

supE44 hsdΔ 5 thi Δ(lac- proAB) F’ [traD36 proAB + lacIq lacZΔM15]

[26]

Top10

F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZ ΔM15 ΔlacX74 deoR nupG recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(strr) endA1 λ-

Gibco BRL

JCB816

MC1000 phoR λ102

[72]

JCB817

JCB816 dsbA::kan1

[72]

JFC383

JCB816 dsbC::kan

J.F. Collet Collection

FED126

MC1000 ΔdsbD

[73]

JFC571

JFC383/pHEL2

This study

JFC572

JCF383/pUWM500

This study

JFC573

JF383/pUWM399 hp0377 +

This study

PR501

JCB817/pHEL2

This study

PR502

JCB817/pUWM399 hp0377 +

This study

PR539

FED126/pUWM399 hp0377 + & pUWM536

This study

PR540

JCB816/pUWM399 hp0377 + & pUWM536

This study

PR580

FED126/pUWM399 hp0377 +

This study

PR581

JCB816/pUWM399 hp0377 +

This study

General cloning/Plasmid vectors

pET28a

Kmr, IPTG inducible

Novagen

pGEM-T Easy

Apr; LacZa

Promega

pBlusescript SK II

Amr, LacZa

Stratagene

pHEL2

Cmr E. coli/H. pylori shuttle vector

[74]

pMPM-K6Ω

Kmr,Spr; ori f1, ori P15A; arabinose inducible

[75]

pRY109

Cmr

[76]

pUOA13

KmR,TetR

[77]

pILL2150

Cmr E.coli/H. pylori shuttle vector; IPTG inducible

[47]

Plasmids for mutagenesis

 

pUWM505

pGEM-T Easy/hp0377::aph

This study

pUWM529

pGEM-T Easy/hp0377::aph

This study

pUWM555

pGEM-T Easy/hp0377::aph

This study

pUWM2019

pBluescript II SK/hp0265::aph

This study

Plasmids for recombinant protein synthesis and purification

 

pUWM518

pET28a/hp0377

This study

pUWM2046

pET28a/hp0377 C89S

This study

pUWM2065

pET28a/hp0377 C92S

This study

pUWM2090

pET24a/hp1227

This study

pET28a-dsbA

pET28a/EcdsbA

J.F. Collet Collection

pET28a-dsbC

pET28a/EcdsbC

J.F. Collet Collection

pUWM525

pET28a/hp0231

[31]

Other plasmids

pUWM500

pHEL2/hp0231

[31]

pUWM336

pHEL2/dsbI

[78]

pUWM399

pHEL2/hp0377

This study

pUWM389

pGEM-T Easy/hp0231

This study

pUWM536

pMPM-K6Ω/hp0265

This study

pUWM543

pGEM-T easy/hp0377

This study

pUWM544

pGEM-T easy/hp0377 without promoter and signal sequence

This study

pUWM2045

pGEM-T easy/hp0377 C89S

This study

pUWM2064

pBluescript II SK/hp0377 C92S

This study

pUWM2073

pBluescript II SK/hp0377 C25A

This study