Skip to main content
Fig. 8 | BMC Microbiology

Fig. 8

From: Helicobacter pylori HP0377, a member of the Dsb family, is an untypical multifunctional CcmG that cooperates with dimeric thioldisulfide oxidase HP0231

Fig. 8

Redox state of HP0377 in wt and mutant: hp0265::aph measured for cells grown in the absence (a) or presence (b) of DTT. Bacterial cultures were treated with 10 % TCA, followed by alkylation with AMS. Cellular proteins including the reduced (red; DTT treated, modified with AMS) and the oxidized (ox; non-modified with AMS) controls were separated by 14 % SDS-PAGE under non-reducing conditions, and Western blot analysis using antibodies against HP0377 was performed. Each lane contains proteins isolated from the same amount of bacteria

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us