Fig. 2From: Helicobacter pylori HP0377, a member of the Dsb family, is an untypical multifunctional CcmG that cooperates with dimeric thioldisulfide oxidase HP0231Oxidase assay of HP0231 towards apocytochrome c (HP1227). Two different SDS PAGEs were run to better visualize the shift between oxidized and reduced forms of proteins. a HP0231-dependent oxidation of reduced apocytochrome c. Lane 1, oxidized HP0231. Lane 2, reduced HP0231, Lane 3, reduced apocytochrome c treated with oxidized HP0231. b HP0231-dependent oxidation of reduced apocytochrome c. Lane 1, oxidized apocytochrome c. Lane 2, reduced apocytochrome c, Lane 3, reduced apocytochrome c treated with oxidized HP0231. Different redox forms were detected by nonreducing SDS-PAGE after AMS treatment, which results in an increase in the molecular mass of reduced proteins by about 0.5 kDa per thiol groupBack to article page