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Table 2 Comparison of virulence of Vibrio anguillarum parent and isogenic mutant strains lacking virulence factors in Galleria mellonella larva and fish models of infection

From: Larva of the greater wax moth, Galleria mellonella, is a suitable alternative host for studying virulence of fish pathogenic Vibrio anguillarum

Knockout mutant Parent Inactivated/missing gene(s) Gene function Virulence change of mutant in fish Virulence change of mutant in larvae Reference
NB10 cured NB10 Virulence plasmid (pJM1) Iron-scavenging function Not done [32]
NB12 NB10 empA Metalloprotease ↓ (Oncorhynchus mykiss) [30]
DM16 NB10 flaA Flagellin protein ↓ (O. mykiss) [27]
KD12 NB10 flaD Flagellin protein ↓ (O. mykiss) n.s. [29]
KD27 NB10 flaE Flagellin protein ↓ (O. mykiss) [29]
S123 M93Sm rtxA Repeat-in-toxin secreted toxin ↓ (Salmo salar) [7]
JR1 M93Sm vah1 Secreted haemolysin ↓ (S. salar) n.s. [28]
S183 M93Sm rtxA and vah1 Secreted toxin and haemolysin ↓ (S. salar) [7]
  1. ↑, virulence of mutant greater than parent; ↓ virulence of mutant less than parent. In the larva model, the virulence change of the mutant compared to the parent was either not significant (n.s.) or significant (p < 0.05; Holm’s corrected). n = 20