Fig. 4
Comparison of affinity response (measured as kd) of five PNG plasma samples. Different bindings to the chip for MSP2-3D7 or MSP2-FC27. N-terminal coupling exploits amine groups in the ligand, C-terminal coupling exploits carboxyl groups. Hexa His–N terminal protein coupling uses the capture of His-tagged constructs at the N-terminal end of the protein, and hexa His-C-terminal protein coupling uses the capture of His-tagged constructs at the C-terminal end of the protein. The box plot values represent the 25th percentile, median, and the 75th percentile. The whisker range determines the 5th and 95th percentiles
