Molecular epidemiology and characterization of an outbreak causing Klebsiella pneumoniae clone carrying chromosomally located bla CTX-M-15 at a German University-Hospital

Table 2 PCR fragment sizes with defined primer combinations as calculated based on the chromosomal insertion*

Forward Primer	Reverse Primer	Resulting PCR-Fragment length in base pairs (bp)
ALA2b	ALA3b	88
ALA2b	P2Bc	290
ALA2b	P2Dc	941
ALA2b	MP4b-F	1516
MP-3 F	DOW2.55	1156
MP-3 F	DOW2.5	1718
MP-3 F	ALA3b	2078
MP-3 F	P2Bc	2280
MP-3 F	P2Dc	2931
MP-3 F	MP4b-F	3506
P1Ac	P2Dc	410
P1Ac	MP4b-F	985
MF-2 F	MF-2R	3435

*When the insertion is not present at the examined locus, no specific PCR fragments should be able to be generated, except for the last primer pair, which should then give a PCR product of 459 base pairs (bp)