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Table 3 Identification of Gram-positive cocci from monomicrobial positive blood cultures by MALDI-TOF (direct and SepsiTyper (ST) methods)*

From: Rapid and reliable identification of Gram-negative bacteria and Gram-positive cocci by deposition of bacteria harvested from blood cultures onto the MALDI-TOF plate

 

No. of isolates

Species

Identification by use of MALDI-TOF MS with score (S) of:

No. of unidentified isolates

No. of misdentified isolates

Total (%)

S < 1.7

1.7 ≤ S < 2

2.0 ≥ S <2.3

S ≥ 2.3

   

Direct

ST

Direct

ST

Direct

ST

Direct

ST

Direct

ST

Direct

ST

S. aureus

1

 

3

 

2

4

 

1

 

1

  

6 (7.5 %)

S. epidermidis

14

11

16

20

3

3

  

3

1

 

1

36 (45 %)

S. capitis

  

1

1

5

5

1

  

1

  

7 (8.9 %)

S. haemolyticus

1

 

1

2

3

3

 

1

1

1

1

 

7 (8.9 %)

S. hominis

2

  

1

4

3

 

2

    

6 (7.5 %)

S. warneri

1

 

1

1

       

1

2 (2.5 %)

Staphylococcus xylosis

  

1

1

        

1 (1.2 %)

Staphylococcus simulans

    

1

1

      

1 (1.2 %)

Staphylococcus sciuri

    

1

1

      

1 (1.2 %)

Staphylococcus pettenkoferi

  

1

1

        

1 (1.2 %)

E. faecium

   

1

4

1

 

1

 

1

1

1

5 (6.3 %)

E. faecalis

    

1

1

1

1

    

2 (2.5 %)

Enterococcus casseliflavus

  

1

  

1

      

1 (1.2 %)

S. oralis group mitis

          

1

1

1 (1.2 %)

Streptococcus anginosus

  

1

1

        

1 (1.2 %)

M. luteus

1

      

1

1

1

  

2 (2.5 %)

Total (%)

20 (25 %)

11 (14 %)

26 (33 %)

29 (36 %)

24 (30 %)

23 (29 %)

2 (2 %)

7 (8 %)

5 (6 %)

6 (8 %)

3 (4 %)

4 (5 %)

80

  1. *Using the results obtained with the routine methods by MALDI-TOF and Vitek 2 as comparators