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Table 1 Identification of Gram-negative bacilli from monomicrobial positive blood cultures by MALDI-TOF (direct method)*

From: Rapid and reliable identification of Gram-negative bacteria and Gram-positive cocci by deposition of bacteria harvested from blood cultures onto the MALDI-TOF plate

 

No. of isolates

  

Species

Identification by use of direct method MALDI-TOF MS with score (S) of:

No. of unidentified isolates

Total (%)

S < 1.7

1.7 ≤ S < 2

2.0 ≥ S <2.3

S ≥ 2.3

Direct MALDI-TOF MS

Routine MALDI-TOF MS

Klebsiella pneumoniae

  

4

40

  

44 (37.3 %)

Escherichia coli

   

23

  

23 (19.5 %)

Enterobacter aerogenes

  

1

4

  

5 (4.3 %)

Enterobacter cloacae

  

1

4

  

5 (4.3 %)

Enterobacter cancerogenus

   

1

  

1 (0.8 %)

Klebsiella oxytoca

   

3

  

3 (2.6 %)

Proteus mirabilis

   

3

  

3 (2.6 %)

Serratia marcescens

  

1

1

  

2 (1.7 %)

Citrobacter freundii

   

1

  

1 (0.8 %)

Pseudomonas aeruginosa

 

3

1

9

  

13 (11 %)

Acinetobacter baumannii

  

10

2

  

12 (10.2 %)

Acinetobacter lwoffii

  

1

1

  

2 (1.7 %)

Pseudomonas stutzeri

  

1

   

1 (0.8 %)

Stenotrophomonas maltophilia

    

1

1

1 (0.8 %)

Bacteroides fragilis

   

1

  

1 (0.8 %)

Fusobacterium nucleatum

1

     

1 (0.8 %)

Total (%)

1 (0.8 %)

3 (2.5 %)

20 (17 %)

93 (78.9 %)

1 (0.8 %)

 

118

  1. *Using the results obtained with the routine methods by MALDI-TOF and Vitek 2 as comparators
  2. No isolates were misidentified. No isolates were unidentified by Vitek 2