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Table 1 Penetration of gentamicin (10 μg/ml) through biofilm of K. pneumoniae B5055

From: Aeromonas punctata derived depolymerase improves susceptibility of Klebsiella pneumoniae biofilm to gentamicin

Biofilm age (Days)

Inhibition zones (mm) observed following penetration of gentamicin through disc placed on

% Retardation

Membrane with no biofilm

Membrane with colony biofilm

1

15 ± 0.06

11 ± 0.14

26.66 ± 0.17

3

15 ± 0.08

7 ± 0.18

53.33 ± 0.18

5

15 ± 0.12

3 ± 0.12

80.06 ± 0.15

7

15 ± 0.05

2 ± 0.13

86.66 ± 0.20

  1. The zone of growth inhibition observed on plates with discs having no biofilms was taken as 100 % penetration and used to determine the percentage retardation of gentamicin through biofilms. Data is presented as mean ± SD. The experiment was performed thrice in triplicates
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BMC Microbiology

ISSN: 1471-2180

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