Fig. 6
Obg is a primarily cytoplasmic protein that predominantly fractionates together with the 50S ribosomal particles. a Samples of isolated subproteome fractions (as indicated; 15 μg) obtained from wild type FA1090 cultured under regular aerobic laboratory growth conditions were separated in 4-20 % gradient gels and probed with either polyclonal rabbit anti-ObgGC antisera or monoclonal mouse anti-MtrE antibodies. b FA1090 cell lysate was subjected to separation on a 10-47 % step sucrose gradient by ultracentrifugation at 174,000 × g for 4 h and monitored by UV absorbance at 254 nm. The positions of the 30S and 50S ribosomal subunits, the 70S monosomes, and polyribosomes are indicated. The level of ObgGC in the collected fractions was detected by immunoblotting with polyclonal rabbit anti-ObgGC antibodies. A representative polyribosome profile and immunoblot are shown