Fig. 5
Expression of ObgGC. The growth of FA1090 (a) and ObgGC amounts (b) were examined during regular aerobic conditions in GCBL by measurements of bacterial turbidity (OD600) and immunoblotting analyses of whole cell lysates every hour. The graph shows means with corresponding SEM from biological triplicate experiments. Samples were matched by equivalent OD600 units and representative immunoblots are shown. Immuoblotting with anti-Ng-MIP antisera was used as a loading control. c The immunoblot probed with anti-ObgGC antisera was scanned and subjected to densitometric analysis. To quantify the intensity of the ObgGC protein bands, the volume tool, local background subtraction, and linear regression methods were used. d The expression of ObgGCwas assessed in whole cell lysates derived from GC cultured on GCB aerobically, in iron-limited conditions, in the presence of 7.5Â % normal human sera, and anaerobically in the presence of nitrite as a terminal electron acceptor. Immunoblotting analyses with anti-TbpB and anti-AniA antisera were used as controls for iron-depleted [54] and anaerobic [55] growth conditions, respectively. Samples of whole-cell lysates were matched by the same OD600 units (40 or 20 as indicated) with the exception of detection of AniA during anaerobic growth conditions, where 0.375 OD600 units were used