Fig. 2
Validation of polyclonal rabbit anti-ObgGC antisera. (a) The polyclonal rabbit anti-ObgGC antibodies were used to probe the whole-cell lysates derived from wild type FA1090 and isogenic P lac ::obg GC as well as purified variants of ObgGC. The bacteria were harvested following 2 h of growth in GCBL with (+) and without (−) 100 μM IPTG, and the samples were matched by equivalent OD600 units. Purified recombinant proteins (40 ng) include wild type ObgGC with N-terminal 6 × His tag, (N-His-ObgGC) and N-His-ObgGC with T192AT193A substitutions. (b) Samples of whole-cell lysates derived from various Neisseria species, as indicated, were harvested from GCB and matched by equivalent OD600 units. All samples were separated in 4-20 % Mini-PROTEAN TGX precast gels, the proteins were transferred onto the nitrocellulose membrane and probed with polyclonal rabbit anti-ObgGC antisera raised against N-His-ObgGC