Phasing amplicon sequencing on Illumina Miseq for robust environmental microbial community analysis

BMC Microbiology

Table 1 Dissimilarity analysis of mock and soil community OTUs among phasing primer sets*^a

Samples	Methods	Jaccard**			Bray-Curtis
Samples	Methods	MRPP	Anosim	Adonis	MRPP	Anosim	Adonis
Mock community	One-step PCR	1	1	n/a	0.001	0.001	0.001
Mock community	Two-steps PCR	1	1	n/a	0.379	0.448	0.374
Neutral black soil	One-step PCR	0.024	0.026	0.027	0.008	0.011	0.002
Neutral black soil	Two-steps PCR	0.142	0.252	0.166	0.341	0.373	0.356

*Data in the table are p values
**For mock community, p value = 1 due to all OTUs were shared by all primer sets
^aBoth the mock community and the neutral black soil were amplified using the 8 phasing primer sets and 3 barcodes (as replicates)

ISSN: 1471-2180