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Table 1 Dissimilarity analysis of mock and soil community OTUs among phasing primer sets*a

From: Phasing amplicon sequencing on Illumina Miseq for robust environmental microbial community analysis

Samples Methods Jaccard** Bray-Curtis
MRPP Anosim Adonis MRPP Anosim Adonis
Mock community One-step PCR 1 1 n/a 0.001 0.001 0.001
Two-steps PCR 1 1 n/a 0.379 0.448 0.374
Neutral black soil One-step PCR 0.024 0.026 0.027 0.008 0.011 0.002
Two-steps PCR 0.142 0.252 0.166 0.341 0.373 0.356
  1. *Data in the table are p values
  2. **For mock community, p value = 1 due to all OTUs were shared by all primer sets
  3. aBoth the mock community and the neutral black soil were amplified using the 8 phasing primer sets and 3 barcodes (as replicates)