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Fig. 4 | BMC Microbiology

Fig. 4

From: Phasing amplicon sequencing on Illumina Miseq for robust environmental microbial community analysis

Fig. 4

Effect of PAS on sequence errors as determined with a mock community. (a) Average sequence error rates of entire sequence reads, the last 97 bases of the forward or reverse reads, and the combined sequence reads for both PAS and non-PAS methods; (b) The percentage of effective sequences for forward and reverse reads, and combined sequences after trimming for both PAS and non-PAS methods (left panel), and the average length of forward and reverse reads for both PAS and non-PAS methods (right panel). All raw sequences were trimmed if the average sequence quality score was < Q30 with a window size of 5. The Student t test was used to test significance: *, p < 0.05; **, p < 0.01; ***, p < 0.001. F, forward; R, reverse; C, combined

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