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Fig. 5 | BMC Microbiology

Fig. 5

From: New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain

Fig. 5

Confocal Laser Scanning Microscopy of biofilm formed by S. pseudintermedius strain DSM 25713. Biofilm was allowed to form for 48 h at 37 °C, in absence of serum, under both (a) static, and (b) dynamic (flow cell chamber) conditions. Static biofilms were further treated for 24 h with increasing gentamicin concentrations (1x-128xMIC). Representative images of biofilm exposed at (c) 1x and (d) 128xMIC gentamicin are shown. Orthogonal images z are projections of x and y planes, collected within the biofilm as indicated by the green and red lines in the top view. Image capture was set for simultaneous visualization of red (Propidium iodide-stained dead cells), green (Syto-9-stained viable cells), and blue (Concanavalin A-stained EPS) fluorescence. Magnification, x100

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