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Fig. 2 | BMC Microbiology

Fig. 2

From: New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain

Fig. 2

Effect of serum and pH on biofilm formation and growth by S. pseudintermedius strain DSM 25713. (a) Serum was tested against biofilm formation at various dilutions (1:2, 1:10, and 1:100), as free or adsorbed to polystyrene, under different pH (5.5, 7.1, and 8.7). Control wells contained bacteria but not serum. Biofilm biomass amount was measured by crystal violet assay, then normalized on bacterial growth by calculating the specific biofilm formation (SBF) index (see Materials and Methods). (b) The effect of free serum against bacterial growth was assessed by measuring OD600 of cell grown in broth following 24 h-incubation. Results are means + SDs (n = 9). *p < 0.05, **p < 0.01, and ***p < 0.001, ANOVA + Newman-Keuls multiple comparison post-test

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