Table 1 Strains and plasmids used in this work

E. faecalis

WT

Fus^r Rif^r; plasmid-free wild-type strain

[35]

ΔelrA

OG1RF ΔelrA

[4]

P⁺-elrA-E

OG1RF P _aphA3 ::elrA-E

This work

P⁺-ΔelrA

OG1RF P _aphA3 ::ΔelrA

This work

P⁺-elrA-ΔelrB-E

OG1RF P _aphA3 ::elrA-ΔelrB-E

This work

P^{+ -ΔelrA-E}

OG1RF P _aphA3 ::ΔelrA-E

This work

E. coli

TG1

supE hsdD5 thi (Δlac-proAB) F’ (traD36 proAB-lacZΔM15)

[36]

BL21(λDE3)

F⁻ ompT gal dcm hsdSB(rB⁻mB⁻) λDE3

[37]

Plasmids

pACYC177

Amp^r, Kan^r, ori p15A

[38]

pET2817

Amp^r, ori colE1, T7 promoter, His-Tag coding sequence

[39]

pGEM-T easy

Amp^r, ori ColE1, linearized with 3’ T overhangs

Promega

pGhost9

Erm^r, ori pWV01, repA(Ts)

[27]

pMV158-GFP

pMV158 with the gene encoding the green fluorescent protein

[24]

pTCV-lac(P _aphA3 )

Tet^r, ori ColE1, ori pAMβ1, lacZ harboring P _aphA3 promoter

[16]

pVE14009

Erm^r, ori pWV01, repA(Ts), with elrA deletion

[4]

pVE14047

Amp^r, pET2817 with 6x His::ElrA

This work

pVE14142

Amp^r, Kan^r, ori p15A, ‘elrR-elrA’ region

This work

pVE14145

Amp^r, Kan^r, ori p15A, ‘elrR-P _aphA3 ::elrA’ region

This work

pVE14146

Erm^r, ori pWV01, repA(Ts), ‘elrR-P _aphA3 ::elrA’ region

This work

pVE14178

Amp^r, ori colE1, with elrA-E deletion

This work

pVE14179

Amp^r, ori colE1, with elrB-E deletion

This work

pVE14450

Erm^r, ori pWV01, repA(Ts), with elrB-E deletion

This work

pVE14455

Amp^r, Kan^r, ori p15A, with P _aphA3 ::elrA-E deletion

This work

pVE14456

Erm^r, ori pWV01, repA(Ts), with P _aphA3 ::elrA-E deletion

This work

pVE14457

Erm^r, ori pWV01, repA(Ts), with P _aphA3 ::elrA deletion

This work