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Table 1 Strains and plasmids used in this work

From: Overexpression of Enterococcus faecalis elr operon protects from phagocytosis

Strain Designation relevant characteristics Source or Reference
E. faecalis   
WT Fusr Rifr; plasmid-free wild-type strain [35]
ΔelrA OG1RF ΔelrA [4]
P+-elrA-E OG1RF P aphA3 ::elrA-E This work
P+elrA OG1RF P aphA3 ::ΔelrA This work
P+-elrA-ΔelrB-E OG1RF P aphA3 ::elrAelrB-E This work
P+ elrA-E OG1RF P aphA3 ::ΔelrA-E This work
E. coli   
TG1 supE hsdD5 thilac-proAB) F’ (traD36 proAB-lacZΔM15) [36]
BL21(λDE3) F ompT gal dcm hsdSB(rBmB) λDE3 [37]
Plasmids   
pACYC177 Ampr, Kanr, ori p15A [38]
pET2817 Ampr, ori colE1, T7 promoter, His-Tag coding sequence [39]
pGEM-T easy Ampr, ori ColE1, linearized with 3’ T overhangs Promega
pGhost9 Ermr, ori pWV01, repA(Ts) [27]
pMV158-GFP pMV158 with the gene encoding the green fluorescent protein [24]
pTCV-lac(P aphA3 ) Tetr, ori ColE1, ori pAMβ1, lacZ harboring P aphA3 promoter [16]
pVE14009 Ermr, ori pWV01, repA(Ts), with elrA deletion [4]
pVE14047 Ampr, pET2817 with 6x His::ElrA This work
pVE14142 Ampr, Kanr, ori p15A, ‘elrR-elrA’ region This work
pVE14145 Ampr, Kanr, ori p15A, ‘elrR-P aphA3 ::elrA’ region This work
pVE14146 Ermr, ori pWV01, repA(Ts), ‘elrR-P aphA3 ::elrA’ region This work
pVE14178 Ampr, ori colE1, with elrA-E deletion This work
pVE14179 Ampr, ori colE1, with elrB-E deletion This work
pVE14450 Ermr, ori pWV01, repA(Ts), with elrB-E deletion This work
pVE14455 Ampr, Kanr, ori p15A, with P aphA3 ::elrA-E deletion This work
pVE14456 Ermr, ori pWV01, repA(Ts), with P aphA3 ::elrA-E deletion This work
pVE14457 Ermr, ori pWV01, repA(Ts), with P aphA3 ::elrA deletion This work