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Table 1 Genes and primers used for MLST

From: The evolution and population structure of Lactobacillus fermentum from different naturally fermented products as determined by multilocus sequence typing (MLST)

Gene Position* Primer Sequence Amplicon size (bp)
clpX 723485 - 724143 clpX/F 5’-CGCACGGAAGCAGAAAC-3’ 659
clpX/R 5’-GAGTCGGTCCCAAACCC-3’
dnaA 410-998 dnaA/F 5’-ACCCGCTCCTGATTTACG-3’ 589
dnaA/R 5’-GCCTCGGTAGCCAGTTTG-3’
dnaK 885345-886078 dnaK/F 5’-GACAACGGTCCGCTCCACT-3’ 734
dnaK/R 5’-TCGGCTTCTTCCTTCTTCTTCT-3’
groEL 394357-395019 groEL/F 5’-CCGACAACGACAAGATGG-3’ 663
groEL/R 5’-CCAAGGCAGGGATAACG-3’
murC 1501098-1500354 murC/F 5’-TTTGAAGCCGACGAATACC −3’ 745
murC/R 5’-CGATGTCCTCGCTACCC-3’
murE 2086362-2085704 murE/F 5’-CTACCGCCAGCACTTCTT-3’ 659
murE/R 5’-GGTCCATCTGGGTGTTTAGC-3’
pepX 1895783-1896506 pepX/F 5’-AAAGAAGACGAGCAACCAACC-3’ 724
pepX/R 5’-CGGAGTCCTTAGTCCCGATT-3’
pyrG 246712-247420 pyrG/F 5’-TCATTGGGTCGGCTGTT-3’ 709
pyrG/R 5’-GGTCCATCCCTTGCTTTTG-3’
recA 605296-605942 recA/F 5’-ATTGCCGACGCCCTGAT-3’ 647
recA/R 5’-TGCGGTTCGCCTTCCTT-3’
rpoB 1719594-1718880 rpoB/F 5’-GAAGTTCCGCCGCTCTA-3’ 715
rpoB/R 5’-GGTCCCATCTGGCATGTAC-3’
uvrC 727309-728056 uvrC/F 5’-TCGTCACCTCCTCCAATAA-3’ 748
uvrC/R 5’-TGGTTCGGTAATCCCTCC-3’
  1. *Positions correspond to complete genome sequence of Lactobacillsu fermentum IFO 3956