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Table 1 Plasmids constructed and used in this study

From: Identification of the replication region in pBCNF5603, a bacteriocin-encoding plasmid, in the enterotoxigenic Clostridium perfringens strain F5603

Plasmid

 

pJIR750

HindIII/EcoRV (2629 bp) fragment of pIP404 replication region

pKZ100

pJIR750 (XmnI/SpeI) Ω pBCNF5603 PCR product (SpeI/SnaBI; 3120 bp) (cop and rep fragment)

pKZ110

pJIR750 (XmnI/SpeI) Ω pCP8533S12 PCR product (BsmI/PflMI; 2632 bp) (rep fragment)

pKZ200

pJIR750 (XmnI/SpeI) Ω pBCNF5603 PCR product (AvrII/BstBI; 5476 bp) (PCP63, soj, parB and PCP03 fragment)

pKZ210

pJIR750 (XmnI/SpeI) Ω pBCNF5603 PCR product (AvrII/HpaI; 2271 bp) (PCP63 fragment)

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BMC Microbiology

ISSN: 1471-2180

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